Growth Hormone (GH1) (21-217) Mouse Monoclonal Antibody [Clone ID: g3H5]

Specifications

Product Data
Clone Name	g3H5
Applications	ELISA, IHC, Neutralize, WB
Recommend Dilution	ELISA. Western blot (1/500-1/1,000). Immunohistochemistry on Paraffin sections (10 µg/ml). This GH antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. Neutralization.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human growth hormone (27-217aa) purified from E. coli
Specificity	The antibody recognizes Growth Hormone (hGH) at aa 27-217.
Isotype	IgG2b, kappa
Formulation	Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Concentration	1mg/ml (determined by BCA assay)
Purification	By protein-G affinity chromatography
Conjugation	Unconjugated
Storage Condition	Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Gene Name	growth hormone 1
Database Link	NP_000506 Entrez Gene 2688 Human UniProt ID P01241
Background	Human growth hormone (hGH, somatotropin), a protein hormone from the anterior lobe of the pituitary gland, comprises a single polypeptide chain of 191 amino acid residues. The 20kDa variant form of human growth hormone (20kDa hGH) presents in extracts from pituitary glands and it differs from the major form of hGH (22kDa, 191 amino acid) by the deletion of amino acid residues 32-46. The growth hormone amino acid sequence varies considerably between species, and non-primate growth hormones have little activity in man. Main functions of hGH and 20kDa hGH are the stimulation of somatic and bone growth, as well as an increase in the size and mass of organs and tissues.
Synonyms	GH1, Growth Hormone, GH, GH-N, Pituitary growth hormone, Growth hormone 1, Somatotropin, GHN, hGH-N
Reference Data

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