MIF Mouse Monoclonal Antibody [Clone ID: 4E4]

Specifications

Product Data
Clone Name	40000
Applications	ELISA, FC, ICC/IF, IF, IHC, WB
Recommend Dilution	ELISA. Western blot: 1/500-1/2,000. Recommended starting dilution is 1/1,000. Flow Cytometry.  Immunofluorescence/Immnunocytochemistry. Immunohistochemistry on Paraffin Sections: 5 µg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human MIF (1-115 aa) purified from E. coli
Specificity	The antibody recognizes Human MIF. Other species not tested.
Isotype	IgG1, kappa
Formulation	Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Concentration	1mg/ml (determined by BCA assay)
Purification	By protein-G affinity chromatography
Conjugation	Unconjugated
Storage Condition	Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Gene Name	macrophage migration inhibitory factor (glycosylation-inhibiting factor)
Database Link	NP_002406 Entrez Gene 4282 Human UniProt ID P14174
Background	The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration.
Synonyms	Macrophage migration inhibitory factor, GLIF, MMIF, MIF, EC 5.3.2.1, Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GIF, Macrophage migration inhibitory factor, macrophage migration inhibitory factor (glycosylation-inhibiting factor)
Reference Data

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