MHC Class II Rat Monoclonal Antibody [Clone ID: YKIX334.2]

Specifications

Product Data
Clone Name	YKIX334.2
Applications	FC, IHC, IP
Recommend Dilution	Immunoprecipitation. Immunohistochemistry on Frozen Sections. Flow Cytometry: Use 10 µl 1/50-1/100 diluted antibody to label 106 cells in 100 µl.
Reactivity	Canine
Host	Rat
Clonality	Monoclonal
Immunogen	Con A activated canine peripheral blood cells. Spleen cells from immunised DA rats were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line.
Specificity	This antibody recognises monomorphic Canine MHC Class II and was classified at the First Canine Leucocyte Antigen Workshop. The antibody immunoprecipitates an antigen of 32/34kD and blocks the proliferation of MHC Class II dependent responses in vitro. In Dogs, MHC Class II is expressed by all peripheral blood mononuclear cells.
Isotype	IgG2a
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Synonyms	MHC Class 2, HLA Class II
Reference Data

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