MHC Class II Rat Monoclonal Antibody [Clone ID: YKIX334.2]

Specifications

Product Data
Clone Name	YKIX334.2
Applications	FC
Recommend Dilution	Flow Cytometry: Use 10 µl Neat antibody to label 106 cells in 100 µl.
Reactivity	Canine
Host	Rat
Clonality	Monoclonal
Immunogen	Con A activated canine peripheral blood cells. Spleen cells from immunised DA rats were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line.
Specificity	This antigen recognises MHC Class II and was classified at the First Canine Leucocyte Antigen Workshop. The antibody immunoprecipitates an antigen of 32/34kD and blocks the proliferation of MHC Class II dependent responses in vitro. In dogs, MHC Class II is expressed by all peripheral blood mononuclear cells.
Isotype	IgG2a
Formulation	PBS Label: FITC State: Liquid purified IgG fraction Stabilizer: 1% BSA Preservative: 0.09% Sodium Azide Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	FITC
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Synonyms	MHC Class 2, HLA Class II
Reference Data

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