ACRV1 Mouse Monoclonal Antibody [Clone ID: Ds-2]

Specifications

Product Data
Clone Name	Ds-2
Applications	IF, WB
Recommend Dilution	Immunocytochemistry; membrane permeabilization (acetone) essential. Western Blotting.
Reactivity	Canine
Host	Mouse
Clonality	Monoclonal
Immunogen	Freshly ejaculated dog sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid, lyophylized and subsequently used for immunization.
Specificity	The antibody reacts with intra-acrosomal proteins (92 and 42 kDa) of sperms.
Isotype	IgG
Formulation	Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 State: Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Protein-A affinity Chromatography; Purity: > 95 % (by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	acrosomal vesicle protein 1
Database Link	Entrez Gene 56 Human UniProt ID P26436
Background	Canine intra-acrosomal sperm protein (92 and 42 kDa) is a testis-specific acrosomal vesicle protein 1 associated with the acrosomal membranes and matrix of the mature sperm.
Synonyms	Acrosomal protein SP-10, SP10, SPACA2, Acrosomal vesicle protein 1, Intra-acrosomal Sperm Protein
Reference Data
Protein Families	Druggable Genome

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