Blood Group Lewis a Antigen Mouse Monoclonal Antibody [Clone ID: 7LE]

Specifications

Product Data
Clone Name	7LE
Applications	ELISA, IHC, WB
Recommend Dilution	Immunohistochemistry (paraffin section): 5 μg/ml (antigen retrieval with sodium citrate). Positive control:colorectal adenocarcinoma. Western Blotting. ELISA.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Mucin isolated by size-exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio's classification)
Specificity	The antibody recognizes Lewis a blood group antigen. Lewis a may be useful for detection of gastrointestinal, pancreatic and colorectal tumors.
Isotype	IgG1
Formulation	Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 State: Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Protein-A affinity chromatography; purity: > 95 % (by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Background	Lewis a blood group antigen is a carbohydrate determinant carried on both glycolipids and glycoproteins, expressed in colonic epithelial cells.
Reference Data

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