CD22 Mouse Monoclonal Antibody [Clone ID: MEM-01]

Specifications

Product Data
Clone Name	MEM-01
Applications	FC, IP, WB
Recommend Dilution	Flow Cytometry: 1/100 - 1/200; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl. Western Blot. Immunoprecipitation: Non-reducing conditions.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Burkitts lymphoma cell line Raji
Specificity	This antibody recognises the CD22 cell surface antigen, a 130/140kD glycoprotein expressed by cells of B lymphocyte lineage. This antibody detects a band of approximately 140kD in cell lysates under non reducing conditions.
Isotype	IgG1
Formulation	PBS, pH7.4 containing 0.09% Sodium Azide State: Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD22 molecule
Database Link	Entrez Gene 933 Human UniProt ID P20273
Background	CD22 is initially expressed cytoplasmically and subsequently on the cell surface during B cell maturation. It is lost upon terminal differentiation to plasma cells.
Synonyms	SIGLEC2, Siglec-2, B-cell receptor CD22, Leu-14, BL-CAM
Reference Data

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