Sirpa Mouse Monoclonal Antibody [Clone ID: OX-41]

Specifications

Product Data
Clone Name	OX-41
Applications	FC, IHC, WB
Recommend Dilution	Western Blot. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. Flow Cytometry: 1/100 - 1/200; Use 10ml of the suggested working dilution to label 10e6 cells in 100µl.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat peritoneal macrophages. Spleen cells from immunised BALB/c mice were fused with the NSO/U mouse myeloma cell line.
Specificity	This antibody recognises CD172a, also known as Signal regulatory protein (SIRP). CD172a is expressed selectively by myeloid cells and by neurons. MRC OX-41 binds to a different epitope on SIRP than ED9. It does not block the interaction of CD172a - CD47.
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide State: Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	signal-regulatory protein alpha
Database Link	Entrez Gene 25528 Rat UniProt ID P97710
Background	Protein tyrosine phosphatases (PTPases) SHP1 and SHP2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPa (1),SHPS1 , MyD1, BIT and p84. SIRPa is a new gene family containing at least fifteen members. SIRPa is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP2. SIRPa has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion and plays a general role in different physiological and pathological processes.
Synonyms	SHP substrate 1, SHPS-1, Sirp-alpha-2, Sirp-alpha-3, MyD-1 antigen, p84, BIT, MFR, MYD1, PTPNS1, SHPS1
Reference Data

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