Cd8b Mouse Monoclonal Antibody [Clone ID: 341]

Specifications

Product Data
Clone Name	341
Applications	FC, IP, WB
Recommend Dilution	Flow Cytometry: 1/100 - 1/200; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl. Immunoprecipitation.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat/mouse T cell hybrids expressing CD8. Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63Ag8.653 myeloma cell line.
Specificity	This antibody recognises the beta chain of the rat CD8 cell surface antigen. This antibody is reported to block proliferative and cytotoxic responses of CD8 +ve effector cells in vitro.
Isotype	IgG1
Formulation	PBS, pH7.4 containing 0.09% Sodium Azide State: Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD8b molecule
Database Link	Entrez Gene 24931 Rat UniProt ID P05541
Background	CD8 is expressed on thymocytes and peripheral cytotoxic/suppressor cells. CD8b is not expressed by NK cells or by intestinal intraepithelial T cells.
Synonyms	CD8B, CD8B1
Reference Data

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