Cd28 Mouse Monoclonal Antibody [Clone ID: E18]

Specifications

Product Data
Clone Name	E18
Applications	FC, FN
Recommend Dilution	Functional Assays. Flow Cytometry: Use 10 µl of 1/50-1/100 diluted to label 106 cells in 100 µl.
Reactivity	Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Alternate immunisation with A20 cells expressing mCD28 and recombinant mCD28Ig. Spleen cells from immunised CD28-/-knockout mice were fused with cells of the X63Ag8.653 myeloma cell line.
Specificity	This antibody recognises CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. Clone E18 has been shown to be able to stimulate murine T lymphocytes in combination with anti-mouse CD3.
Formulation	PBS, pH 7.4 State: Low Endotoxin State: Liquid purified IgG fraction Stabilizer: None Preservative: None
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at -20°C. Avoid repeated freezing and thawing.
Gene Name	CD28 antigen
Database Link	Entrez Gene 12487 Mouse UniProt ID P31041
Background	Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95% of CD4+ cells and 50% of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells. The first is mediated via the T cell receptor following its interaction with antigenic peptide MHC complexes, and the second is delivered by accessory or costimulating molecules through their counter receptors on T lymphocytes. CD28 bears structural homology to CTLA 4 which is expressed at very low levels on the surface of CD4+ and CD8+ peripheral blood cells only following activation. CD28 is the natural receptor for the B7/BB 1 ligand (CD80) a 55-60 kDa glycoprotein which is expressed on activated B lymphocytes, on dendritic cells and on interferon gamma treated monocytes. The binding of B7 1/BB 1 molecules to CD28 is involved in T lymphocyte activation and in the initiation and maintenance of chronic inflammation.
Synonyms	TP44
Note	Endotoxin Level: less than 0.01 EU/µg
Reference Data

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