CD61 / ITGB3 Hamster Monoclonal Antibody [Clone ID: HM beta 3.1]

Specifications

Product Data
Clone Name	HM beta 3.1
Applications	FC
Recommend Dilution	Flow Cytometry (Neat-1/10): Use 10 µl of of the suggested working dilution to label 10e6 cells in 100 µl. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors.
Reactivity	Mouse, Rat
Host	Hamster
Clonality	Monoclonal
Immunogen	Mouse alpha 5 beta 3 protein purified from the mouse hybridoma 2B4. Spleen cells from immunised Armenian hamsters were fused with cells of the P3U1 mouse myeloma cell line.
Specificity	This antibody recognises the integrin beta 3 subunit (CD61). Clone HM beta 3-1 is reported to partially inhibit the binding of CD61 to fibronectin. We recommend the use of SM2240LE for this purpose.
Isotype	IgG
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer. Label: FITC State: Liquid purified IgG fraction. Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	FITC
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Gene Name	integrin beta 3
Database Link	Entrez Gene 16416 Mouse UniProt ID O54890
Background	CD61 is a 90kD a type I membrane protein, which is expressed primarily on megakaryocytes, platelets, monocytes, macrophages, granulocytes and endothelial cells. CD61 associates with either the alpha IIb integrin (CD41) or the alpha V integrin (CD51) to form the platelet glycoprotein complex IIb/IIIa and the vitronectin receptor (VNR) respectively. The heterodimers formed with CD61 are receptors for a variety of ligands including fibrinogen, fibronectin, von Willebrands factor (vWF), vitronectin and thrombospondin.
Synonyms	Integrin beta-3, GP3A, GPIIIa
Reference Data

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