TNF Mouse Monoclonal Antibody [Clone ID: CC327]

Specifications

Product Data
Clone Name	CC327
Applications	ELISA, FC
Recommend Dilution	Flow Cytometry: Membrane permeabilisation is required for this application. ELISA: This reagent may be used as a Capture antibody in ELISA assays for bovine TNF alpha in combination with SM2228B as Detection reagent.
Reactivity	Bovine
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant bovine TNF alpha.
Specificity	This antibody clone CC327 recognizes Bovine TNF alpha.
Isotype	IgG2b
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction from tissue culture supernatant
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	Entrez Gene 280943 Bovine UniProt ID Q06599
Background	Tumor Necrosis Factor alpha (TNF alpha) is a 17.5kDa cytokine, expressed by many different stimulated cell types including monocytes, macrophages, endothelial cells, fibroblasts and both T and B-lymphocytes. The production of TNF alpha is induced by a variety of factors, dependant upon cell type and includes bacterial toxins, IL-1, PDGF, IFN-beta, NGF, Oncostatin M and viral infections. The presence of TNF alpha is responsible for diverse immunomodulatory, anti-tumour and toxic effects and under certain conditions is also capable of self-stimulation and inhibition.
Synonyms	TNF, TNF-a, TNFA, TNFSF2, Cachectin
Reference Data

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