Aurora A (AURKA) Mouse Monoclonal Antibody [Clone ID: 35C1]

Specifications

Product Data
Clone Name	35C1
Applications	IF, IP, WB
Recommend Dilution	Immunoprecipitation. Western Blot: 1/500 - 1/1000; this antibody detects a band of approximately 46kDa in human HeLa and mouse M-ICc12 cell lysates. Immunofluorescence. Recommended Positive Control: Human 293 and mouse LLC1 cell lines.
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Aurora-A. Spleen cells from immunised Balb/c were fused with cells of the SP2/0-Ag14 mouse myeloma cell line.
Specificity	This antibody recognises Aurora-A kinase. Clone 35C1 specifically recognises an epitope within the non-catalytic N-terminal domain of Aurora-A. Clone 35C1 does not inhibit Aurora-A kinase activity.
Formulation	PBS, pH7.4 containing 0.09% Sodium Azide State: Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	aurora kinase A
Database Link	Entrez Gene 6790 Human UniProt ID O14965
Background	Aurora-A kinase is a 46kDa serine/threonine kinase involved in mitotic spindle assembly. Aurora-A is reported to be overexpressed in many epithelial cancers and is thought to play an important role in tumourigenesis.
Synonyms	AURKA, AIK1, ARK1, AURA, BTAK, STK15, STK6, Aurora/IPL1-related kinase 1, AURORA2
Reference Data

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