LOC100507703 Mouse Monoclonal Antibody [Clone ID: BB7.2]

Specifications

Product Data
Clone Name	BB7.2
Applications	FC, IHC, IP
Recommend Dilution	Immunoprecipitation. Immunohistochemistry on Frozen Sections. Recommended Positive Control: Tonsil. Flow Cytometry: Use 10 µl of 10 µg/ml diluted antibody to label 10e6 cells or 100 µl whole blood.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Papain solubilized HLA-A2. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Specificity	This antibody recognises the HLA-A2 histocompatability antigen. The epitope recognised by this antibody has been studied extensively and would appear to include the carboxy-terminus of the alpha-2 helix and a turn on one of the underlying beta strands. This antibody may be used for the Flow Cytometric detection of HLA-A2 expression and has also been used for immunoaffinity purification of HLA-A2 molecules.
Isotype	IgG2b
Formulation	PBS containing 0.09% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID P01892
Background	Human leukocyte antigens are highly polymorphic proteins that are involved in the presentation of antigens to the T cell receptor. There are two classes of HLA antigens, class I (HLA A, HLA B and HLA C) and class II (HLA D).
Synonyms	HLAA2, MHC class I antigen A-2
Reference Data

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