Integrin alpha E (ITGAE) Mouse Monoclonal Antibody [Clone ID: LF61]

Specifications

Product Data
Clone Name	LF61
Applications	FC
Recommend Dilution	Flow Cytometry: Use 5 µl of neat-1/10 diluted antibody to label 106 cells in 100 µl.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Hairy cell leukaemia cells. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSO myeloma cell line.
Specificity	This antibody recognizes the Human CD103 cell surface antigen. Other species not tested.
Isotype	IgG1
Formulation	PBS Label: FITC State: Liquid purified IgG faction Stabilizer: 0.5% BSA Preservative: 0.09% Sodium Azide Label: Fluorescein Isothiocyanate Isomer 1 Absorption emission: 490 nm / 525 nm
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	FITC
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Gene Name	integrin subunit alpha E
Database Link	Entrez Gene 3682 Human UniProt ID P38570
Background	CD103/Integrin alpha E is a new member of the integrin series of adhesion molecules containing the beta7 subunit. This antigen defines a developmentally important subset of T cells, namely mucosal T cells including all IEL (intraepithelial lymphocytes) and ~20% of lamina propria T cells. Expression of CD103 is more restricted outside these mucosal organs, appearing at lower levels on T cell subsets of the lymph node, dendritic epidermis and periphery. In non-epithelial CD103+ T cells there is a bias toward expression on CD8+ cells. Integrin alpha E is a heterodimer comprising a 150kDa alpha chain and a 120kDa beta chain. Integrin alpha E is expressed by approximately 1% of peripheral blood lymphocytes, activated T lymphocytes and by hairy cell leukaemia cells. The antigen is also expressed by intraepithelial lymphocytes. It has recently been shown to be identical to the alpha E integrin.
Synonyms	Integrin alpha-E, Integrin alpha-IEL, HML-1 antigen, ITGAE
Reference Data

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