CD45 (PTPRC) (CD45RB) Mouse Monoclonal Antibody [Clone ID: MEM-55]

Specifications

Product Data
Clone Name	MEM-55
Applications	FC, IHC, IP, WB
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 106 cells in 100 µl. Immunoprecipitation. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 1/50-1/200. Protein digestion pretreatment of paraffin sections not required. Antigen retrieval using heat treatment prior to staining of paraffin sections not required.
Reactivity	Human, Primate, Rabbit
Host	Mouse
Clonality	Monoclonal
Specificity	This antibody recognizes the isoform of the leucocyte common antigen encoded by exon B and stains B cells, most T cells and monocytes. Clone MEM-55 immunoprecipitates bands of 190, 200 and 205kDa.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	protein tyrosine phosphatase, receptor type C
Database Link	Entrez Gene 5788 Human UniProt ID P08575
Background	CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
Synonyms	PTPRC, Leukocyte common antigen, L-CA, T200
Reference Data

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