HLAG (HLA-G) Mouse Monoclonal Antibody [Clone ID: MEM-G/1]

Specifications

Product Data
Clone Name	MEM-G/1
Applications	IHC, WB
Recommend Dilution	Western Blotting. Recommended dilution 1-2 μg/ml. Immunohistochemistry on Paraffin Sections: 1/60-1/100 Incubation Time: 1 hour at RT. Positive tissue: Human placenta - extravillous cytotrophoblast. Staining technique: Standard ABC technique. Pretreatment: Heat retrieval in 0.01 M citrate buffer (4x2 min. in microwave oven).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human HLA-G denaturated heavy chain
Specificity	The antibody reacts with denaturated HLA-G heavy chain. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.
Isotype	IgG1
Formulation	Phosphate buffered saline (PBS), pH~7.4 with 15 mM Sodium Azide State: Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
Concentration	lot specific
Purification	Affinity Chromatography on Protein-A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. Do Not freeze.
Gene Name	major histocompatibility complex, class I, G
Database Link	Entrez Gene 3135 Human UniProt ID P17693
Background	HLA G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of a heavy chain (with a1, a2, a3 domains) and a light chain (beta 2 microglobulin). Alternate splicing provides both soluble and membrane bound forms of HLA G, with two soluble isoforms and four membrane bound isoforms. HLA G may be part of the fetal protection system and is expressed on fetal derived placental cells.
Synonyms	HLA-6.0, HLAG, MHC class I antigen G
Reference Data

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