Myeloperoxidase (MPO) Mouse Monoclonal Antibody [Clone ID: 2C7]

Specifications

Product Data
Clone Name	2C7
Applications	FC
Recommend Dilution	Flow Cytometry: Use 10 µl of Neat-1/10 diluted antibody to label 106 cells in 100 µl. Membrane permeabilisation is required for this application.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human myeloperoxidase. Spleen cells from immunised mice were fused with cells of the mouse X63 AG8-653 myeloma cell line.
Specificity	This antibody recognises myeloperoxidase (MPO), an important component of azurophilic granules in neutrophils. Negative Species: Rat.
Formulation	PBS, pH 7.4 Label: FITC State: Liquid purified IgG fraction Stabilizer: 1% BSA Preservative: 0.09% Sodium Azide Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	FITC
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Gene Name	myeloperoxidase
Database Link	Entrez Gene 4353 Human UniProt ID P05164
Background	Human myeloperoxidase (MPO) is a dimeric protein composed of two heavy subunits (53 kDa) and two light subunits (15 kDa). Each MPO molecule contains two prosthetic porphyrins which play an important role in the catalytic cycle. Molecular weights for MPO isoforms from pools of normal human samples range from 114,000 to 140,000 daltons reflecting a heterogeneous mixture of isoforms when assayed under non-reducing conditions of SDS-PAGE. Often MPO from a single donor will yield a homogenous preparation reflecting a single isoform. The carbohydrate component of MPO, consisting of mannose, glucose and N-acetylglucosamine residues is 2.5%. MPO is inhibited by azide and other compounds. MPO is stored in primary granules of neutrophils and serves as a bactericidal agent in that MPO catalyzes the production of hypochlorous acid (HOCl), a powerful oxidant. HOCl is derived from chloride ion (Cl-) and hydrogen peroxide (H2O2). In a number of inflammatory situations, MPO is released into the extracellular matrix where its measurement can be used as an indication of neutrophil activation.
Synonyms	MPO
Reference Data

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