Ubiquitin (UBB) Mouse Monoclonal Antibody [Clone ID: 1B4-UB]

Specifications

Product Data
Clone Name	1B4-UB
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA. Western Blot. Flow Cytometry: 1/10 - 1/50; Membrane permeabilisation is required for this application. Immunohistochemistry on frozen sections: 1/500 - 1/1000. Immunohistochemistry on paraffin sections: 1/500 - 1/1000; This product does not require protein digestion pre-treatment of paraffin sections; This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Recommended Positive Control: Human hypophysis.
Reactivity	Bovine, Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Ubiquitin from bovine erythrocytes conjugated to bovine gamma Globulin. Spleen cells from immunised BALB/c mouse were fused with cells of the mouse PAI myeloma cell line.
Specificity	This antibody recognises ubiquitin.
Isotype	IgG1
Formulation	PBS, pH7.4 containing 0.09% Sodium Azide State: Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	ubiquitin B
Database Link	Entrez Gene 7314 Human UniProt ID P0CG47
Synonyms	UBB, Polyubiquitin-B
Reference Data

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