alpha Defensin 1 (DEFA1) Mouse Monoclonal Antibody [Clone ID: DEF-3]

Specifications

Product Data
Clone Name	DEF-3
Applications	IHC
Recommend Dilution	Immunohistochemistry on Frozen Sections: 0.2 µg/ml (1/1000). Immunohistochemistry on Paraffin Sections: 0.5 µg/ml (1/400), Proteinase K pretreatment for antigen retrieval is recommended. Positive Control: Human tonsil. Has been discribed to work in FACS with permeabilized cells.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Native defensins. The epitope has not been further characterized.
Specificity	Several Defensins are recognized. This antibody recognises a family of Cyclic Peptides in Neutrophils. Human: Defensin 1-3 (HNP-1 to HNP-3) in Human Neutrophils. Synthetic Defensin-1 and -2 (Bachem, Bubendorf CH) stain also positively. Not tested with Defensin-4. A side reaction to elastin has been observed in humans. Other: not tested. Defensins have been described in rabbits (NP-1 to NP-5, NP3a = Corticostatin), and in mice (cryptodin).
Isotype	IgG1
Formulation	PBS, pH 7.2, containing 5 mg/ml BSA and 0.09% sodium azide State: Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore with 0.5 ml distilled water.
Concentration	0.2 mg/ml (after reconstitution)
Purification	Affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store the original vial at 2-8°C. Freeze stock solution or aliquots thereof at -20°C. Avoid repeated freezing and thawing. Do not freeze working dilutions.
Gene Name	defensin alpha 1
Database Link	Entrez Gene 728358 HumanEntrez Gene 1667 Human UniProt ID P59665
Background	Defensins are a family of cyclic peptides containing 29-35 amino acids (MW < 3500) which tend to form aggregates. Four of these peptides are described in humans (HNP-1 to 4), six in rabbits (NP-1 to 5). Synonyms are MCP-1 for NP-1, MCP-2 for NP-2 and corticostatin for NP-3. Biological Functions: Various functions have been described for defensins. They are antibacterial, antifungal, chemotactic for monocytes, inhibitory for ACTH-induced corticosteroid synthesis and cytotoxic for cells. Defensins are inhibited by glucosaminoglycans (self protection for cells) and high concentrations of Ca2+ ions.
Synonyms	DEFA1, DEF-A1, DEFA-1, DEF1, DEFA2, MRS, DEF-1, DEFA-2, DEF-A2, HNP-1, HP-1, HP1, HNP1, Neutrophil defensin 1, DEFA3, DEFA-3, DEF-A3, DEF3, DEF-3, HNP-3, HP-3, HP3, HNP3, Neutrophil defensin 3, Defensin alpha 1, Defensin alpha 3
Reference Data
Protein Families	Druggable Genome

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