Cytokeratin 14 (KRT14) Mouse Monoclonal Antibody [Clone ID: LL002]

Specifications

Product Data
Clone Name	LL002
Applications	FC, IF, IHC, WB
Recommend Dilution	Western Blot. Immunofluorescence. Flow Cytometry: Use 10 µl of 1/100 diluted antibody to label 106 cells in 100 µl (Membrane permeabilisation is required). Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 1/200. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer is recommended for this purpose. Enzyme digestion is not recommended. Recommended Positive Control: Skin, tonsil or squamous cell carcinoma.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Last 15 C-terminal residues of Human Cytokeratin 14 conjugated to Thyroglobulin.
Specificity	This antibody recognises Cytokeratin 14, a type I intermediate filament, expressed by stratifying epithelial cells and can be used to distinguish these cell types from simple epithelial cells, which do not express Cytokeratin 14.
Isotype	IgG3
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Ion Exchange Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	keratin 14
Database Link	Entrez Gene 3861 Human UniProt ID P02533
Background	Cytokeratin is a type I intermediate filament, expressed by stratifying epithelial cells and can be used to distinguish these cell types from simple epithelial cells, which do not express cytokeratin 14. Keratin 14 is a member of the keratin family, the most diverse group of intermediate filaments. It is usually found as a heterotetramer with two molecules of keratin 5, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. Keratin 14 has been studied as a prognostic marker in breast cancer.
Synonyms	Cytokeratin-14, CK14, Keratin 14, K14, KRT14
Reference Data

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