Fibroblasts / Epithelial Cells Mouse Monoclonal Antibody [Clone ID: D7-FIB]
CAT#: SM1214P
Fibroblasts / Epithelial Cells mouse monoclonal antibody, clone D7-FIB, Purified
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CNY 7538.00
货期*
4周
规格
Cited in 3 publications. |
Specifications
Product Data | |
Clone Name | D7-FIB |
Applications | FC, IHC, IP |
Recommend Dilution | Flow Cytometry: Use 10 µl of a 1/50-1/200 diluted antibody to label 106 cells in 100 µl. This product is routinely tested on the KG1 cell line. Immunohistochemistry on Frozen Tissues or Cells: 1/100. This antibody is reported to be sensitive to formaldehyde fixation and tissue prossesing. Use ice-cold methanol for 5 min or acetone as fixative. Does not work on Paraffin Sections. Immunoprecipitation. |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | Human foreskin Fibroblasts. Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2 myeloma cell line. |
Specificity | SM1214P recognizes a 112 kD molecule expressed on the cell surface of human fibroblasts and epithelial cells. In peripheral blood the antibody stains myeloid cells and a very small number of lymphocytes. Studies upon the antigen have shown it to be sensitive to SDS but resistant to trypsin, tunicamycin and collagenase. In immunohistochemical studies the antibody has also been found to bind to epithelium, myoepithelium, smooth muscle cells and some leucocytes. D7-FIB has been shown to be useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes (Van Osch et al., 2001). Negative Species: Mouse, Rat. |
Isotype | IgG2a |
Formulation | PBS containing 0.09% Sodium Azide as preservative State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant |
Concentration | lot specific |
Purification | Affinity Chromatography on Protein G |
Conjugation | Unconjugated |
Storage Condition | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Background | A fibroblast is a connective-tissue cell of mesenchymal (somewhat undifferentiated) origin that secretes proteins from which the extracellular fibrillar matrix of connective tissue forms. Epithelial cells are cells that cover the surface of the body and line its cavities. |
Synonyms | Fibroblast Marker, Fibroblasten |
Reference Data |
Citations (3)
The use of this Antibodies has been cited in the following citations: |
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Comparative Analysis of Peptide Composition and Bioactivity of Different Collagen Hydrolysate Batches on Human Osteoarthritic Synoviocytes
,null,
Scientific Reports
,PubMed ID 30531866
|
Correlation of hypoxia status with radiosensitizing effects of sodium glycididazole: A preclinical study
,null,
Oncology Letters
,PubMed ID 29616117
|
Characterization of human lung tumor-associated fibroblasts and their ability to modulate the activation of tumor-associated T cells
,Nazareth MR, Broderick L, Simpson-Abelson MR, Kelleher RJ, Yokota SJ, Bankert RB,
J. Immunol.
,PubMed ID 17442937
|
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