Fibroblasts / Epithelial Cells Mouse Monoclonal Antibody [Clone ID: D7-FIB]

CAT#: SM1214P

Fibroblasts / Epithelial Cells mouse monoclonal antibody, clone D7-FIB, Purified



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CNY 7538.00


货期*
4周

规格
    • 200 ug

Cited in 3 publications.

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Specifications

Product Data
Clone Name D7-FIB
Applications FC, IHC, IP
Recommend Dilution Flow Cytometry: Use 10 µl of a 1/50-1/200 diluted antibody to label 106 cells in 100 µl.
This product is routinely tested on the KG1 cell line.
Immunohistochemistry on Frozen Tissues or Cells: 1/100.
This antibody is reported to be sensitive to formaldehyde fixation and tissue prossesing. Use ice-cold methanol for 5 min or acetone as fixative.
Does not work on Paraffin Sections.
Immunoprecipitation.
Reactivity Human
Host Mouse
Clonality Monoclonal
Immunogen Human foreskin Fibroblasts.
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2 myeloma cell line.
Specificity SM1214P recognizes a 112 kD molecule expressed on the cell surface of human fibroblasts and epithelial cells. In peripheral blood the antibody stains myeloid cells and a very small number of lymphocytes.
Studies upon the antigen have shown it to be sensitive to SDS but resistant to trypsin, tunicamycin and collagenase. In immunohistochemical studies the antibody has also been found to bind to epithelium, myoepithelium, smooth muscle cells and some leucocytes.
D7-FIB has been shown to be useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes (Van Osch et al., 2001).
Negative Species: Mouse, Rat.
Isotype IgG2a
Formulation PBS containing 0.09% Sodium Azide as preservative
State: Purified
State: Liquid purified IgG fraction from Tissue Culture Supernatant
Concentration lot specific
Purification Affinity Chromatography on Protein G
Conjugation Unconjugated
Storage Condition Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Background A fibroblast is a connective-tissue cell of mesenchymal (somewhat undifferentiated) origin that secretes proteins from which the extracellular fibrillar matrix of connective tissue forms. Epithelial cells are cells that cover the surface of the body and line its cavities.
Synonyms Fibroblast Marker, Fibroblasten
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (3)

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