CD62P (SELP) Mouse Monoclonal Antibody [Clone ID: AK-6]

Specifications

Product Data
Clone Name	AK-6
Applications	FC, IHC, WB
Recommend Dilution	Western Blot. Flow Cytometry: Use 10 µl of 1/10-1/100 diluted antibody to label 106 cells in 100 µl. Immunohistochemistry on Frozen Sections: 1/1000-1/5000. Immunohistochemistry on Paraffin Sections: 1/500-1/2000. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections but staining may be enhanced using Sodium Citrate buffer pH6.0. Recommended Positive Control Tissue: Tonsil.
Reactivity	Human, Monkey
Host	Mouse
Clonality	Monoclonal
Immunogen	Human platelet membrane glycoproteins.
Specificity	This antibody recognises the CD62P glycoprotein, a 140kD molecule expressed by activated platelets and endothelial cells.
Isotype	IgG1
Formulation	PBS, pH 7.4 without preservatives State: Azide Free State: Liquid purified IgG fraction Stabilizer: None
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at -20°C Only. Avoid repeated freezing and thawing.
Gene Name	selectin P
Database Link	Entrez Gene 6403 Human UniProt ID P16109
Background	CD62P (P-selectin) is an adhesion glycoprotein that is expressed on platelets and endothelial cells upon their activation. Interaction between CD62P and its mucin-like ligand PSGL-1 (P-selectin glycoprotein ligand-1) expressed on the microvilli of most leukocytes supports leukocyte rolling along postkapillary venules at the earliest time of inflammation. Both CD62P and PSGL-1 are extended glycoproteins that form homodimers. CD62P dimerization is probably mediated through interactions of the transmembrane domains and stabilizes leukocyte tethering and rolling, probably by increasing rebinding within a bond cluster. The antigen is expressed in the surface of activated platelets and also on endothelial cells both of blood and lymph vessels.
Synonyms	SELP, GMRP, GRMP, PADGEM, GMP-140, LECAM3
Reference Data

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