CD40 Mouse Monoclonal Antibody [Clone ID: LOB7/6]

Specifications

Product Data
Clone Name	LOB7/6
Applications	FC, IF, IHC, IP
Recommend Dilution	Flow Cytometry: Use 10 µl of 20 µg/ml diluted antibody to label 106 cells in 100 µl. Immunohistochemistry on Frozen sections: 1-10 mg/ml. Immunohistochemistry on Paraffin sections: 1-10 µg/ml. This product requires protein digestion pre-treatment of paraffin embedded sections prior to staining eg. pronase or trypsin. Recommended Positive Control: Human Tonsil. Immunoprecipitation.
Reactivity	Canine, Human
Host	Mouse
Clonality	Monoclonal
Immunogen	CD40 Ig(Fc) fusion protein containing the EC region of human CD40 and Fc region of Human IgG. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Specificity	This antibody Clone LOB7/6 recognizes the CD40 cell surface antigen.
Isotype	IgG2a
Formulation	Tris buffered saline containing 0.09% Sodium Azide as preservative State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD40 molecule
Database Link	Entrez Gene 958 Human UniProt ID P25942
Background	CD40 is a 48kD glycoprotein expressed by B lymphocytes and weakly by some monocytes. CD40 is involved in the process of B cell selection in germinal centres, and is vital in T cell-B cell interactions.
Synonyms	TNFRSF5, CDw40, Bp50
Reference Data

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