CD35 (CR1) Mouse Monoclonal Antibody [Clone ID: E11]

Specifications

Product Data
Clone Name	E11
Applications	FC, IHC, WB
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/50-1/200 diluted antibody to label 106 cells or 100 µl whole blood. Immunohistochemistry on Frozen Section: 1-10 µg/ml. Immunohistochemistry on Paraffin Section: 1-10 µg/ml. Requires antigen retrieval using heat treatment prior to staining of Paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose. Recommended Positive Control: Human Tonsil. Western Blotting (Ref.3).
Reactivity	Human, Monkey, Baboon
Host	Mouse
Clonality	Monoclonal
Immunogen	Human acute monocytic leukaemia cells. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Specificity	This antibody is specific for CD35, a single chain cell surface glycoprotein which exists in four allotypic forms (A,B,C,D) of 190kD, 220kD, 160kD and 250kD respectively.
Isotype	IgG1
Formulation	PBS State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	complement component 3b/4b receptor 1 (Knops blood group)
Database Link	Entrez Gene 1378 Human UniProt ID P17927
Background	CD35 is a single chain cell surface glycoprotein which exists in four allotypic forms (A,B,C,D) of 190kD, 220kD, 160kD and 250kD respectively. The molecule is expressed by granulocytes, monocytes, B cells and some T cells. Functionally CD35 acts as a receptor for the C3b component of complement.
Synonyms	Complement receptor type 1, CR1, C3b/C4b receptor
Reference Data

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