CD28 Rat Monoclonal Antibody [Clone ID: YTH913.12]

Specifications

Product Data
Clone Name	YTH913.12
Applications	FC, FN, IHC
Recommend Dilution	Flow Cytometry: 1/50 - 1/100; Use 10ml of the suggested working dilution to label 10e6 cells in 100µl. Immunohistochemistry on frozen sections. Functional Assays: Stimulation of MLR response.
Reactivity	Human
Host	Rat
Clonality	Monoclonal
Immunogen	Human peripheral blood T-cells. Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag 1.2.3 rat myeloma cell line.
Specificity	This antibody recognises the CD28 cell surface antigen, a 44kD glycoprotein expressed by T lymphocytes. The antibody enhances the MLR response. It has been reported that this clone also recognises an epitope of CD28 expressed by NK cells, which is not recognised by clones of 9.3 or CD28.2.
Formulation	PBS, pH7.4 State: Low Endotoxin State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Gene Name	CD28 molecule
Database Link	Entrez Gene 940 Human UniProt ID P10747
Background	Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95% of CD4+ cells and 50% of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells. The first is mediated via the T cell receptor following its interaction with antigenic peptide MHC complexes, and the second is delivered by accessory or costimulating molecules through their counter receptors on T lymphocytes. CD28 bears structural homology to CTLA 4 which is expressed at very low levels on the surface of CD4+ and CD8+ peripheral blood cells only following activation. CD28 is the natural receptor for the B7/BB 1 ligand (CD80) a 55-60 kDa glycoprotein which is expressed on activated B lymphocytes, on dendritic cells and on interferon gamma treated monocytes. The binding of B7 1/BB 1 molecules to CD28 is involved in T lymphocyte activation and in the initiation and maintenance of chronic inflammation.
Synonyms	TP44
Note	Endotoxin Level: less than 0.01 EU/µg
Reference Data

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