CD18 (ITGB2) Rat Monoclonal Antibody [Clone ID: YFC118.3]

Specifications

Product Data
Clone Name	YFC118.3
Applications	FC, IHC, IP
Recommend Dilution	Flow Cytometry: 1/50 - 1/100; use 10 µl of the suggested working dilution to label 10e6 cells in 100µl. Immunoprecipitation. Immunohistochemistry on frozen sections: 1/1000 - 1/5000. Recommended positive control: Human Tonsil.
Reactivity	Canine, Human
Host	Rat
Clonality	Monoclonal
Immunogen	Human neutrophils. Spleen cells from immunised LOU rats were fused with cells of the rat Y3/Ag.1.2.3 myeloma cell line.
Specificity	This antibody recognises the CD18 cell surface antigen, the integrin b2 subunit. This antibody blocks CD18 mediated homotypic adhesion.
Isotype	IgG2b
Formulation	PBS, pH7.4 containing 0.09% Sodium Azide State: Purified State: Liquid purified IgG
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	integrin subunit beta 2
Database Link	Entrez Gene 3689 Human UniProt ID P05107
Background	CD18 is expressed by the majority of leucocytes.
Synonyms	Integrin beta-2, MFI7
Reference Data

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