Human Lambda Light Chain Mouse Monoclonal Antibody [Clone ID: Mc24-IC6]

Specifications

Product Data
Clone Name	Mc24-IC6
Applications	FC
Recommend Dilution	Flow cytometry.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified human IgG. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
Specificity	This antibody recognises lambda light chains, and shows no cross- reactivity to kappa light chains or to immunoglobulin heavy chains.
Isotype	IgG1
Formulation	PBS, pH7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin Label: PE State: Lyophilized purified IgG Label: R. Phycoerythrin (RPE)
Reconstitution Method	Restore with 1 ml distilled water
Purification	Affinity chromatography on Protein A
Conjugation	PE
Storage Condition	Prior to and following reconstitution store the antibody at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Background	All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).
Reference Data

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