Human Lambda Light Chain Mouse Monoclonal Antibody [Clone ID: Mc24-IC6]

Specifications

Product Data
Clone Name	Mc24-IC6
Applications	FC
Recommend Dilution	Flow cytometry: use neat antibody to label 10e6 cells or 10 µl washed whole blood.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified human IgG. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
Specificity	This antibody recognises lambda light chains, and shows no cross- reactivity to kappa light chains or to immunoglobulin heavy chains.
Isotype	IgG1
Formulation	PBS, pH7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin Label: FITC State: Liquid purified IgG fraction. Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity chromatography on Protein A
Conjugation	FITC
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Background	All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).
Reference Data

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