MHC Class II RT1B (monomorphic) Mouse Monoclonal Antibody [Clone ID: OX-6]

Specifications

Product Data
Clone Name	OX-6
Applications	FC, IHC, WB
Recommend Dilution	Western Blot. Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10e6 cells in 100 µl. Immunohistochemistry on Frozen Sections. Immunohistochemsitry on Paraffin Sections: 1/50-1/100. This product does not require antigen retrieval using heat treatment or protein digestion prior to staining of formalin-fixed paraffin-embedded sections, but results may be enhanced with heat treatment using 0.01M citrate buffer pH6. This clone has also been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde).
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat thymocyte membrane glycoprotein. Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Specificity	This antibody recognises a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. This antibody cross reacts with certain mouse strains of MHC haplotypes k and s. Analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. This antibody does not react with the BDIX rat strain. Expression is polymorphic in mice.
Isotype	IgG1
Formulation	PBS, pH 7.2 containing 0.09% Sodium Azide as preservative State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Synonyms	HLA Class II, MHC Class 2 RT1B
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.