Dendritic cells + Interdigitating cells Rat Monoclonal Antibody [Clone ID: MIDC-8]

Specifications

Product Data
Clone Name	MIDC-8
Applications	IHC
Recommend Dilution	Immunohistochemitsry on Frozen Tissues (use at 5 µg/ml, 1/80). Does not react on routinely processed Paraffin Sections. Suggested Positive Control: Mouse spleen.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Mouse lymph node tissue.
Specificity	MIDC-8 is identifies dendritic leukocytes including epidermal Langerhans cells, veiled cells and interdigitating cells in secondary lymphoid organs, the medulla of the thymus and its in vitro counterparts. In combination with clone NLDC-145 (cat.no. BM4017) it is very useful for the isolation of dendritic cells from the thymus. Double staining with MIDC-8 and NLDC-145 allows a more precise characterization of cells to be studied. Whereas MIDC-8 recognizes very specific cytoplasmic components, NLDC-145 stains cytoplasm and cell surface. The MIDC-8 antigen is localized in the cytoplasm as distinct spots near the nucleus. Immunohistological analyses indicate that the antigen recognized by MIDC-8 and NLDC-145 may be biochemically related. Staining of Isolated Cells: The antigen is found in dendritic cells. MIDC-8 does not stain thymic epithelial cells which are the major contaminants in thymic dendritic cell preparations. The antigen is also absent in blood cells, bone marrow cells or macrophages isolated from the peritoneal cavity. Staining of Tissues: The antigen is found in interdigitating cells in T cell dependent areas of secondary lymphoid organs, the medulla of thymus, veiled cells, and very weakly in Langerhans cells.
Isotype	IgG2a
Formulation	PBS, pH 7.2 containing 10 mg/ml BSA as stabilizer and 0.01% Thimerosal as a preservative. State: Purified State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore with 0.5 ml distilled water.
Concentration	0.4 mg/ml
Purification	Affinity chromatography.
Conjugation	Unconjugated
Storage Condition	Store the original vial at 2-8°C. Freeze stock solution or aliquots thereof at -20°C. Avoid repeated freezing and thawing.
Synonyms	Dendritic cell marker
Reference Data

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