Thy1 Mouse Monoclonal Antibody [Clone ID: OX-7]

Specifications

Product Data
Clone Name	OX-7
Applications	FC, IHC, IP, WB
Recommend Dilution	Western Blot. Immunoprecipitation. Immunohistochemistry on Frozen Sections. Flow Cytometry: Use 10 µl of 1/50-1/100 dilted antibody to label 10e6 cells in 100 µl. This product is routinely tested in flow cytometry on Rat thymocytes.
Reactivity	Guinea Pig, Mouse, Rabbit, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat Thy 1 antigen.
Specificity	This antibody recognises Rat and Mouse CD90, also known as Thy1.1, expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, T lymphocytes (Mouse), immature B cells (rat) and connective tissues. Since Thy1.1 is a monomorphic determinant in Rat but polymorphic in Mice, clone MRC OX-7 reacts with Thy1.1 Mice e.g. AKR and FVB Mice, but not Thy1.2 Mice such as CBA and BALB/c. Clone MRC OX-7 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic neurons, using fluorescence microscopy. (5) This clone has been reported to induce glomerular nephritis in Wistar Rats. (8) Affinity of the Fab' of MRC OX-7: Rat Thy1: 3 x 10e9me1 Mouse Thy1.1: 3 x 10e8m-1 (1).
Isotype	IgG1
Formulation	PBS, pH7.2 containing 0.09% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	thymus cell antigen 1, theta
Database Link	Entrez Gene 21838 Mouse UniProt ID P01831
Background	Thy1.1 is expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, T lymphocytes (mouse), immature B cells (rat) and connective tissues.
Synonyms	Thy-1, THY1, CDw90
Reference Data

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