CD45 / LCA (CD45R) Rat Monoclonal Antibody [Clone ID: RA3-6B2]

CAT#: SM034PT

CD45 / LCA (CD45R) rat monoclonal antibody, clone RA3-6B2, Purified



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CNY 2,570.00


货期*
4周

规格
    • 25 ug

Product images

Specifications

Product Data
Clone Name RA3-6B2
Applications FC, IHC, IP
Recommend Dilution Flow Cytometry: Use 10 µl of 1/100-1/200 diluted antibody to label 10e6 cells in 100 µl.
Immunoprecipitation.
Immunohistochemistry on Frozen and Paraffin Sections (PLP fixed tissue-See Ref.3).
Reactivity Feline, Human, Mouse
Host Rat
Clonality Monoclonal
Immunogen Mouse pre-B tumour cells (RAW112).
Spleen cells from immunised Lewis rats were fused with cells of the rat S194/5 XX0.BU-1 myeloma cell line.
Specificity This antibody reacts with CD45R a form of the CD45 antigen expressed by B cells and lytically active subsets of NK cells and non-MHC restricted CTLs.
Clone RA3-6B2 immunoprecipitates the high molecular weight form of CD45 (220kD).
Formulation PBS, pH 7.4 containing 0.09% Sodium Azide as preservative.
State: Purified
State: Liquid purified IgG fraction.
Concentration lot specific
Purification Affinity Chromatography on Protein G.
Conjugation Unconjugated
Storage Condition Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Gene Name protein tyrosine phosphatase, receptor type, C
Background CD45R is a member of the protein tyrosine phosphatase (PTP) family and a major cell surface glycoprotein. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45R represents a restricted form of the CD45 family which primarily recognizes only cells of B lineage from proB cell through mature B lymphocytes and, prior to the availability of anti CD19 MAbs, was commonly used as a pan B cell marker. It also reacts with certain activated T cells, as well as non MHC restricted lytically active lymphokine activated killer (LAK) cells. CD45R contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T and B cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. CD45R also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.
Synonyms PTPRC, Leukocyte common antigen, L-CA, T200
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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