CD11b Rat Monoclonal Antibody [Clone ID: M1/70.15]

Specifications

Product Data
Clone Name	M1/70.15
Applications	FC, FN, IHC, IP
Recommend Dilution	Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections. Immunoprecipitation. Flow Cytometry: 1/50-1/100 (routinely tested on Mouse peritoneal macrophages). Functional Assays: Clone M1/70.15 has been reported to block iC3b binding to its receptor. Immunofluorescence.
Reactivity	Human, Mouse, Rabbit
Host	Rat
Clonality	Monoclonal
Immunogen	T cell enriched splenocytes from B10 mice. Spleen cells from immunised DA rats were fused with cells of the NS1/1.Ag4.1 mouse myeloma cell line.
Specificity	This antibody recognises the CD11b cell surface antigen (also known as the alpha M integrin chain or MAC-1), a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages.
Isotype	IgG2b
Formulation	State: Low Endotoxin State: Liquid purified Ig fraction without preservatives.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Database Link	UniProt ID P05555
Background	The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.
Synonyms	ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1
Note	Endotoxin Level: < 0.01EU/µg
Reference Data

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