Cd8a Rat Monoclonal Antibody [Clone ID: KT15]

Specifications

Product Data
Clone Name	KT15
Applications	FC
Recommend Dilution	Flow Cytometry: Use 10 µl of Neat-1/10 diluted antibody to label 10e6 cells in 100µl. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	T cell clone, C6. Spleen cells from a Spraque Dawley rat immunised with the C6 T cell clone were fused with cells of the NSO mouse myeloma cell line.
Specificity	This antibody reacts to Ly2.1 and Ly2.2 cells. Reacts with the CD8 alpha chain (non polymorphic). Weakly cytotoxic.
Isotype	IgG2a
Formulation	PBS containing 0.09% Sodium Azide as presrevative and 1% BSA as stabilizer. Label: PE State: Lyophilized purified IgG fraction. Label: R. Phycoerythrin (RPE)
Reconstitution Method	Restore with distilled water: SM011R with 1.0 ml SM011RT with 0.25 ml SM011RX with 5.0 ml
Conjugation	PE
Storage Condition	Prior to and following reconstitution store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Gene Name	CD8 antigen, alpha chain
Database Link	Entrez Gene 12525 Mouse UniProt ID P01731
Background	The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
Synonyms	CD8 alpha chain, CD8A, MAL
Reference Data

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