TIP120A (CAND1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	Western blot (1:500-1:1,000). Immunoprecipitation: The antibody immunoprecipitates in vitro translated protein and protein from transfected cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related proteins has not been tested. A 125.4 kDa band corresponding to human CAND2 is detected. CAND2 is specifically expressed in muscle and heart tissue. ELISA: (1:2,000-1:10,000).
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1130-1143 of Human CAND2/TIP120B (C-terminal) coupled to KLH.
Specificity	This antibody is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. It reacts with human, rat and mouse CAND2/TIP120B. Cross reactivity does occur with human, rat and mouse CAND1/TIP120A. Cross reactivity with CAND2 from other sources is not known.
Formulation	State: Serum State: Liquid (sterile filtered) containing 0.01% (w/v) Sodium Azide as preservative.
Concentration	lot specific
Purification	Delipidation and defibrination.
Conjugation	Unconjugated
Storage Condition	Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Gene Name	cullin associated and neddylation dissociated 1
Database Link	Entrez Gene 55832 Human UniProt ID Q86VP6
Background	CAND2 is also known as TIP120B, and TATA-binding protein-interacting protein 120B. While both CAND1 (TIP120A) and CAND2 (TIP120B) are TATA-binding proteins and form complexes with various nuclear proteins involved in the control of eukaryotic gene transcription, CAND2 (TIP120B) is expressed specifically in muscle and heart tissue. This is contrary to the ubiquitous expression of CAND1 (TIP120A). TIP120 homologs exist in various higher eukaryotes including D. melanogaster, C. elegans, and A. thaliana. TIP120B is 60% identical in amino acid sequence to TIP120A.
Synonyms	KIAA0829, TIP120, TIP120A, p120 CAND1
Reference Data

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