ZNF148 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	This polyclonal antibody reacts human ZBP-89 in a variety of immunological assays including western blot and ELISA. This antibody is likely functional in immunohistochemistry and immunoprecipitation. Recommended dilutions Immunoblotting: 1:5,000. A band at approximately 89 kDa corresponding to human ZBP-89 is detected. Human monocytes or macrophages or nuclear extracts from PMA treated U937 cells can be used as a positive control. ELISA: 1:10,000 - 1:30,000.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Purified full length ZBP-89 recombinant protein expressed in E.coli
Specificity	This polyclonal antibody is specific for ZBP-89.
Formulation	State: Serum State: Liquid (sterile filtered) Ig fraction containing 0.09% (w/v) Sodium Azide
Concentration	lot specific
Purification	Delipidation and defibrination
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted (in aliquots) at -20°C. Avoid cycles of freezing and thawing.
Gene Name	zinc finger protein 148
Database Link	Entrez Gene 7707 Human UniProt ID Q9UQR1
Background	The GI tract abundantly expresses growth factors many of which bind and activate the EGF receptor present on mucosal cells. One such factor is the zinc finger protein (ZBP-89) that binds to a GC-rich DNA element in the gastrin promoter and confers EGF responsiveness. The fulllength protein functions as a repressor of growth factor signals regulating the gastrin promoter. Several other growth related promoters are also regulated by ZBP-89. ZBP-89 is one of a family of related transcriptional regulators. It has been reported in recent studies that ZBP-89 regulates growth in part by stimulating the cyclin-dependent kinase inhibitor, p21waf1, in a butyrate-dependent manner through recruitment of the histone acetyl transferase p300. Moreover, ZBP-89 triggers growth arrest in a p53-dependent manner by preventing nuclear export of p53. ZBP-89 also induces apoptosis, but this process occurs independent of p53.
Synonyms	ZBP89
Reference Data

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