AVD Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, IHC
Recommend Dilution	Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays.
Reactivity	Chicken
Host	Rabbit
Clonality	Polyclonal
Immunogen	Avidin [Hen Egg White].
Specificity	This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and Avidin.
Formulation	0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative. Label: FITC State: Lyophilized purified Ig fraction Label: Diaminotriazynlaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption emission: 495 nm / 528 nm Molar radio: 3.0 moles DTAF per mole of Rabbit IgG
Reconstitution Method	Restore with 2.0 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Immunoaffinity chromatography
Conjugation	FITC
Storage Condition	Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
Gene Name	avidin
Database Link	Entrez Gene 396260 Chicken UniProt ID P02701
Background	The biological function of avidin is not known. It is a homotetrameric glycoprotein found in chicken egg white, and forms a strong non covalent specific complex with biotin (one molecule of biotin per subunit of avidin).
Synonyms	AVD
Reference Data

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