KTI3 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	Western blot: 1/1,000-1/5,000. ELISA: 1/2,000-1/10,000. Immunohistochemistry: 1/200-1/1,000.
Reactivity	Soy Bean
Host	Rabbit
Clonality	Polyclonal
Immunogen	Trypsin inhibitor from soybean
Specificity	This antibody detects soybean Trypsin inhibitor. Cross reactivity against Trypsin inhibitor from other sources may occur but have not been specifically determined. Immunoelectrophoresis give a single precipitin arc against anti-peroxidase and anti-rabbit serum as well as purified and partially purified Trypsin inhibitor [soybean].
Formulation	0.02 M Potassium phosphate, 0.15 M Sodium hloride, pH 7.2 Label: HRP State: Lyophilized IgG fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!) Label: Horseradish peroxidase
Reconstitution Method	Restore with 0.1 ml of deionized water or equivalent.
Concentration	lot specific
Purification	Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	HRP
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	UniProt ID P01070
Background	Soybean trypsin inhibitor inhibits trypsin and to a lesser extent chymotrypsin and plasmin. Soybean trypsin inhibitor will also inhibit other proteases by a mechanism similar to trypsin. It has inhibitory effects towards plasma kallikrein and coagulation Factor Xa. However, soybean trypsin inhibitor will not inhibit metalloproteases, tissue-based kallikrein, acid proteases, or thio proteases. Anti-trypsin inhibitor antibody is ideal for investigators involved in cell signaling, biochemistry and signal transduction research.
Reference Data

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