SOD1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and most Immunological methods requiring high titer and specificity. Recommended Dilutions: ELISA: 1/6,000-1/30,000. Western Blot: 1/600-1/3,000. QC: This product has been assayed against 1.0 ug of Superoxide Dismutase [Bovine erythrocytes] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:300 to 1:1500 of the reconstitution concentration is suggested for this product.
Reactivity	Bovine
Host	Rabbit
Clonality	Polyclonal
Immunogen	Superoxide Dismutase from Bovine erythrocytes.
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Superoxide Dismutase [Bovine Erythrocytes]. Cross reactivity against Superoxide Dismutase from other tissues and species may occur but have not been specifically determined.
Isotype	IgG
Formulation	0.01M Sodium Phosphate, 0.14M Sodium Chloride, pH 7.4 State: Serum State: Lyophilized purified Ig fraction Stabilizer: None Preservative: 0.01% Sodium Azide
Reconstitution Method	Restore with 2.0 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Prepared from monospecific antiserum by a delipidation and defibrination.
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	Entrez Gene 281495 Bovine UniProt ID P00442
Synonyms	SOD-1, CuZn-SOD, CuZnSOD, IPOA, ALS1
Reference Data

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