Pancreatic Islet Lysate Dog Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Suitable for Immunoblotting (Western or Dot blot) and ELISA. Recommended Dilutions: This product has been assayed against 1.0 ug of Pancreatic Islet Lysate [Porcine Pancreas] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Dog IgG [H&L] (Goat) and o-Phenylenediamine (OPD) as a substrate. A working dilution of 1:50 to 1:100 is suggested for this product. This product has been assayed against 1.5 ug of Pancreatic Islet Lysate [Porcine Pancreas] per lane by Immunoblot (Western) under reducing conditions of SDS-PAGE prior to transfer to nitrocellulose and detection using Peroxidase conjugated Affinity Purified anti-Dog IgG [H&L] (Goat) and Diaminobenzidine (DAB) as a substrate. A working dilution of 1:50 to 1:200 is suggested for this product.
Reactivity	Porcine
Host	Dog
Clonality	Polyclonal
Immunogen	Sonicated crude membrane fraction of porcine pancreatic islets of langerhans cells.
Specificity	This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Dog Serum. Cross reactivity was determined by immunoblot (western) to be minimal against porcine kidney and porcine liver cell lysates.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative. State: Ig Fraction State: Liquid (sterile filtered) purified Ig fraction.
Concentration	lot specific
Purification	Multi-step process.
Conjugation	Unconjugated
Storage Condition	Store vial at -70°C prior to opening. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.