GALM Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	Western blot: 1/500-1/5,000. Immunoprecipitation: 1/100. ELISA: 1/5,000-1/20,000. This product has been assayed against 1.0 µg of Mutarotase [porcine kidney] in a standard sandwich ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/500 to 1/1,500 of the reconstitution concentration is suggested.
Reactivity	Porcine
Host	Rabbit
Clonality	Polyclonal
Immunogen	Mutarotase from porcine kidney
Specificity	This product detects porcine Mutarotase / Aldose 1-epimerase. Cross reactivity against Mutarotase from other tissues and species may occur but have not been specifically determined. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum as well as purified and partially purified porcine Mutarotase.
Formulation	0.02M Potassium phosphate, 0.15M Sodium chloride, pH 7.2 Label: HRP State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!) Label: Horseradish peroxidase
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	HRP
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	Entrez Gene 399536 Pig UniProt ID Q9GKX6
Background	Mutarotase converts alpha-aldose to the beta-anomer. It is active on D-glucose, L-arabinose, D-xylose, D-galactose, maltose and lactose
Synonyms	GALM, BLOCK25, Galactose mutarotase
Reference Data

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