ansB Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Western blot: 1/500. ELISA: 1/1,000. L-asparaginase antibody has been assayed against 1.0 µg of asparaginase in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/3,200 to 1/12,800 of the reconstitution concentration is suggested.
Reactivity	Escherichia coli
Host	Rabbit
Clonality	Polyclonal
Immunogen	Asparaginase from E. coli
Specificity	This antibody detects asparaginase [E. coli]. Cross reactivity against asparaginase from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis give a single precipitin arc against anti-biotin, anti-rabbit serum as well as purified and partially purified asparaginase [E. coli].
Formulation	0.01 M Potassium phosphate, 0.14 M Sodium Chloride, pH 7.4 Label: Biotin State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Sodium Azide
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	UniProt ID P00805
Reference Data

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