D Amino Acid Oxidase (DAO) Sheep Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	ELISA: 1/5,000-1/20,000 Western blot: 1/500-1/5,000 Immunoprecipitation: 1/100 This product has been assayed against 1.0 ug of D-amino-acid oxidase [pig kidney] in a standard sandwich ELISA using peroxidase conjugated affinity purified anti-sheep IgG [H&L] (goat) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested.
Reactivity	Porcine
Host	Sheep
Clonality	Polyclonal
Immunogen	D-amino-acid oxidase from porcine kidney
Specificity	This antibody detects porcine D-amino-acid oxidase. Immunoelectrophoresis give a single precipitin arc against purified and partially purified D-amino-acid oxidase [pig kidney].
Formulation	0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2, with 0.01% Sodium azide as preservative. State: Serum State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore with 2.0 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Prepared from monospecific antiserum by a delipidation and defibrination.
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	D-amino-acid oxidase
Database Link	UniProt ID P14920
Background	This gene encodes the peroxisomal enzyme D-amino-acid oxidase. The enzyme is a flavoprotein which uses flavin adenine dinucleotide (FAD) as its prosthetic group. Its substrates include a wide variety of D-amino-acids, but it is inactive on the naturally occurring L amino acids. Its biological function is not known; it has been suggested that it is involved in acid base balance in the kidney or it could act as a detoxifying agent which removes D-amino-acids accumulated during aging, or it may be a fossil enzyme without a current function.
Synonyms	DAO, DAMOX, DAAO, OXDA, DAO1
Reference Data

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