ADA Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation, Conjugation and most immunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 0.3 ug of Adenosine Deaminase [Calf Spleen] by immunoblot using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) (R1364HRP) and DAB as a substrate at room temperature. A working dilution of 1:5,000 to 1:10,000 of the reconstitution concentration is suggested for this product.
Reactivity	Bovine
Host	Rabbit
Clonality	Polyclonal
Immunogen	Adenosine Deaminase from Calf Spleen.
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Adenosine Deaminase [Calf Spleen]. Cross reactivity against Adenosine Deaminase from other tissues and species are not specifically determined, but are very probable due to high sequence homologie (human >90%, mouse/rat 85%).
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide. State: Serum State: Lyophilized purified Ig faction.
Reconstitution Method	Restore with 2.0 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Prepared from monospecific antiserum by a delipidation and defibrination.
Conjugation	Unconjugated
Storage Condition	Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20°C or below. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Database Link	Entrez Gene 280712 Bovine UniProt ID P56658
Synonyms	Adenosine aminohydrolase, ADA
Reference Data

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