Human Lambda Light Chain Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC
Recommend Dilution	Immunohistochemistry on formalin-fixed paraffin embedded sections: dilute 1:250-1:1000 in an ABC method (incubation time: 30 minutes at room temperature). Recommended positive control: Tonsil
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Lambda light chains isolated from pooled urine of patients with Bence Jones proteinuria
Specificity	This antibody reacts with free as well as bound Lambda light chains. Contaminating antibodies have been removed by solid phase absorption. Cellular localization: Cytoplasmic
Formulation	State: Purified State: Liquid purified Ig fraction containing sodium azide as preservative
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).
Reference Data

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