TIE1 Mouse Monoclonal Antibody [Clone ID: 6F12]

Specifications

Product Data
Clone Name	6F12
Applications	ELISA, FC, WB
Recommend Dilution	ELISA: 1-15 μg/ml. Western blot: 1-2 μg/ml. FACS: 1-5 μg/ml. Cell sorting: 2-5 μg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human soluble extracellular TIE-1 protein.
Specificity	This unconjugated monoclonal antibody will detect native TIE-1 in ELISA experiments and on the surface of different cell types.
Isotype	IgG1
Formulation	PBS Label: Biotin State: Lyophilized purified IgG fraction Stabilizer: BSA (50X) Preservative: 0.02% Sodium Azide
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml
Purification	Protein G Chromatography
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	tyrosine kinase with immunoglobulin like and EGF like domains 1
Database Link	Entrez Gene 7075 Human UniProt ID P35590
Background	The soluble receptor protein consists of the full extracellular domain (Met1-Glu749). The recombinant mature TIE-1/Fc is a disulfide-linked homodimeric protein. Human TIE-1/Fc monomer has a calculated molecular mass of approximately 105kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 125kDa protein in SDS-PAGE under reducing conditions.TIE-1 (tyrosine kinase with Ig and EGF homology domains 1) and TIE-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Human TIE-1 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Whereas two ligands have been described for TIE-2 [angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2)], so far no ligand was found for TIE-1.
Synonyms	TIE, Tie-1
Reference Data

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