Eph receptor A2 (EPHA2) Mouse Monoclonal Antibody [Clone ID: GM5H5]

Specifications

Product Data
Clone Name	GM5H5
Applications	ELISA, FC, IF, WB
Recommend Dilution	Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400. Flow cytometry: 1.2 µg/10e6 cells. Immunofluorescence: 1 µg/10e6 cells. ELISA (detection): With clone LA-4E7-D2 as capture antibody.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Genetic immunisation with cDNA encoding Human EphA2
Specificity	Recognizes EphA2 receptor tyrosine kinase (EphA2).
Isotype	IgG1
Formulation	Phosphate buffered saline, pH 7.2 State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	EPH receptor A2
Database Link	Entrez Gene 1969 Human UniProt ID P29317
Background	EphA2 (Eph receptor tyrosine kinase A2) belongs to the Eph tyrosine receptor familiy, the largest receptor tyrosine kinase family of transmembrane proteins. It encodes a 130 kDA transmembrane protein which is primarily found in adult human epithelial cells (1). Eph receptors and their ephrin ligands are important mediators of cell-cell communication and play roles in embryonic patterning, neuronal targeting, and vascular development during normal embryogenesis (2,3). The Eph family of receptor tyrosine kinases is frequently overexpressed in a wide variety of cancers and tumor cell lines. In particular, EphA2 is overexpressed in prostate, lung and colon cancers and 40% of breast cancers and it represent an attractive potential target for drug design (3,4).
Synonyms	Ephrin type-A receptor 2, Epithelial cell kinase, Eph receptor A2
Note	SDS-PAGE analysis: The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Figure.3).
Reference Data
Protein Families	Druggable Genome, Protein Kinase, Transmembrane
Protein Pathways	Axon guidance

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