CD49b (ITGA2) Human Monoclonal Antibody [Clone ID: B4F12]

Specifications

Product Data
Clone Name	B4F12
Applications	ELISA, FC, IF
Recommend Dilution	IF, ELISA, preferential detection of activated platelet by flow cytometry, platelet aggregation studies. Usage recommendation: *This monoclonal antibody may be used between 5-25μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before use.
Reactivity	Human
Host	Human
Clonality	Monoclonal
Immunogen	CD40 activated and EBV immortalized human B lymphocytes
Specificity	GPIa-IIa preferentially on acitvated platelets.
Isotype	IgG1
Formulation	100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8 State: Purified
Concentration	lot specific
Purification	QMA Hyper D ion exchange chromatography
Conjugation	Unconjugated
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Gene Name	integrin subunit alpha 2
Database Link	Entrez Gene 3673 Human UniProt ID P17301
Background	Neonatal AlloImmune Thrombocytopenia (NAIT) is the commonest cause of severe isolated thrombocytopenia in the foetus and newborn. In Caucasians, 97% of NAIT are caused by HPA-1a or HPA- 5b alloantibodies. B4F12 was isolated after combination of CD40 activation and Epstein-Barr virus transformation of B cells isolated from a woman with high titre of anti -HPA-5b alloantibodies. B4F12 human monoclonal antibody recognizes GPIa-IIa preferentially on activated human platelets (Mérieux Y, Rigal D, Pin JJ, XVIIth Regional Congress, International Society of Blood Transfusion, Madrid 2007 ; Guillot-Chene P, Lebecque S, Rigal D, Annales Pharmaceutiques Françaises, 06/2009, 67 (3) 182-6).
Synonyms	Integrin alpha-2, ITGA-2, VLA-2 alpha, VLA2, GPIa, Collagen Receptor
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.