HIV-1 (gp120/160) Mouse Monoclonal Antibody [Clone ID: 109A2.01]

Specifications

Product Data
Clone Name	109A2.01
Applications	FC, FN, IF
Recommend Dilution	DDX1303P-50 / DDX1303P-100 Purified: ImmunoFluorescence, Flow cytometry, HIV-gp120 binding studies. DDX1303A647-50 / DDX1303A647-100 Alexa- fluor®647 (on request): ImmunoFluorescence, Flow cytometry, HIV-gp120 binding studies. DDX1303B-50 / DDX1303B-100 Biotin (on request): ImmunoFluorescence, Flow cytometry, HIV-gp120 binding studies. Usage recommendation: *This monoclonal antibody may be used between 5-25 μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before using.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	gp160 (HIVIIIB from Biomérieux), gp120 (BAL from NIH), and CHO-NL4.3 cells
Specificity	HIV gp120 and gp160.
Isotype	IgG1
Formulation	Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8. Coupled: 100 μg in 200μl / 50 μg in 100 μl Tris PBS 50% glycerol. Label: Alexa Fluor 647
Concentration	lot specific
Purification	QMA Hyper D ion exchange chromatography
Conjugation	Alexa Fluor 647
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Background	HIV env gene encodes for a primary env product (gp160) which is cleaved into gp120 and gp41. gp120 is anchored to the viral membrane, via non-covalent bonds with the transmembrane glycoprotein, gp41. Mice were immunized with gp160 and gp120 recombinant proteins and the CHO-NL4.3 line expressing gp120 and the extracellular domain of gp41.
Synonyms	HIV-I, HIV1, Human immunodeficiency virus type 1
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.